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B B + $ F 9 R R R v R R R R PC$v R r 0 + R 0 " R R R R + B K : S u p p l e m e n t a r y f i g u r e 1 : I L - 2 1 R i s u n d e t e c t a b l e o n p a n c r e a t i c b- c e l l s b y f l o w c y t o m e t r y A ) I L - 2 1 R e x p r e s s i o n o n b- c e l l s ( I n s u l i n + C D 4 5 - S S C h i g h ) a n d l e u k o c y t e s ( C D 4 5 + S S C l o w I n s u l i n - ) d e r i v e d f r o m t h e p a n c r e a s o f a 9 w k o l d p r e - d i a b e t i c N O D m o u s e w a s d e termined by three-step staining protocol in flow cytometry as described ADDIN EN.CITE Jin200476976976917Jin, H.Carrio, R.Yu, A.Malek, T. R.Distinct activation signals determine whether IL-21 induces B cell costimulation, growth arrest, or Bim-dependent apoptosisJ Immunol.J Immunol.657-65.1731AnimalsAntigens, CD40/physiologyApoptosis/*drug effectsApoptosis Regulatory ProteinsB-Lymphocytes/*drug effects/immunology/physiologyCarrier Proteins/*physiologyCell CycleCells, CulturedInterleukin-21 Receptor alpha SubunitInterleukins/*pharmacologyLipopolysaccharides/pharmacology*Lymphocyte ActivationMembrane Proteins/*physiologyMiceMice, Inbred BALB CMice, Inbred C57BLProto-Oncogene Proteins/*physiologyRatsReceptors, Antigen, B-Cell/physiologyReceptors, Interleukin/physiologyReceptors, Interleukin-212004[24]. Specific and control staining are represented by the tinted area and dashed line, respectively.
Supplementary figure 2: IL-21R knockout mice contain unaltered numbers of nave and effector CD4+ T cells in pancreatic draining ly m p h n o d e s A ) P a n c r e a t i c L N C D 4 + T c e l l s w e r e a n a l y z e d b y f l o w c y t o m e t r y f o r t h e a c t i v a t i o n m a r k e r s C D 6 2 L , C D 4 4 ( t o p p a n e l s ) , a n d C D 1 2 2 ( I L - 2 R b) a n d C D 1 2 7 ( I L - 7 R a) . R e p r e s e n t a t i v e p s e u d o c o l o r d e n s i t y F A C S p l o t ( g a t e d o n C D 4 + C D 3 + T c e l l s ) f r o m o n e o f t h r e e . 2 | ~ " N P X ^ n p H
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